mapk erk inhibitor pd98059  (Cell Signaling Technology Inc)

Journal: Antioxidants

Article Title: NADPH Oxidase 5 (NOX5) Upregulates MMP-10 Production and Cell Migration in Human Endothelial Cells

doi: 10.3390/antiox13101199

Figure Lengend Snippet: p38 MAPK, MEK/ERK, and JNK pathways participate at different levels in NOX5-dependent regulation of MMP-10: ( A ) MMP-10 mRNA levels of tHMock and tHNOX5 cell lines after 24 h of incubation with 5 μM 219138-24-6 (p38 MAPK inhibitor, ip38), PD98059 (MEK/ERK inhibitor, iERK), or JNK-IN-8 (JNK inhibitor, iJNK) ( n = 6). ( B ) MMP-10 protein in the conditioned medium of tHMock and tHNOX5 cell lines after 24 h of incubation with 5 μM 219138-24-6 (ip38), PD98059 (iERK), or JNK-IN-8 (iJNK) ( n = 6). tHMock: stable cell line transfected with pcDNA3.2-Mock. tHNOX5: stable cell line transfected with pcDNA3.2-NOX5. n.s.: not significant differences, * p < 0.05, *** p < 0.001, **** p < 0.0001. Data are presented as median and IQR.

Article Snippet: The p38 MAPK inhibitor (ab145872, Abcam ® , Waltham, MA, USA), JNK MAPK inhibitor (JNK-IN-8, SML1246, Sigma Aldrich), and ERK MAPK inhibitor (PD98059, 9900S, Cell Signaling Technology, Danvers, MA, USA) were used at final concentrations of 5 μM in the cell medium.

Techniques: Incubation, Stable Transfection, Transfection

Journal: Antioxidants

Article Title: NADPH Oxidase 5 (NOX5) Upregulates MMP-10 Production and Cell Migration in Human Endothelial Cells

doi: 10.3390/antiox13101199

Figure Lengend Snippet: NOX5 enhances MMP-10 promoter activity via the JNK pathway and a functional AP-1 site: ( A ) MMP-10 promoter activity of tHMock and tHNOX5 cell lines at baseline and stimulated with 0.25 μM Ang II ( n = 6). ( B ) MMP-10 promoter activity of tHMock and tHNOX5 cell lines after 24 h of incubation with 5 μM 219138-24-6 (p38 MAPK inhibitor, ip38), PD98059 (MEK/ERK inhibitor, iERK), or JNK-IN-8 (JNK inhibitor, iJNK) ( n = 6). ( C ) Schematic representation of MMP-10 promoter constructions used and quantification of MMP-10 promoter activity of tHMock and tHNOX5 cell lines transfected with MMP-10 promoter constructions ( n = 6). CREB: cAMP response element binding protein (CREB) putative union site. AP-1: activator protein-1 (AP-1) putative union site. Crosses indicate site-directed mutations in the putative union sites. tHMock: stable cell line transfected with pcDNA3.2-Mock. tHNOX5: stable cell line transfected with pcDNA3.2-NOX5. ns: not significant differences, * p < 0.05 vs. tHMock Ctrl, *** p < 0.001, **** p < 0.0001, # p < 0.05 vs. tHNOX5 Ctrl cells. Data are presented as median and IQR or as mean ± SEM.

Article Snippet: The p38 MAPK inhibitor (ab145872, Abcam ® , Waltham, MA, USA), JNK MAPK inhibitor (JNK-IN-8, SML1246, Sigma Aldrich), and ERK MAPK inhibitor (PD98059, 9900S, Cell Signaling Technology, Danvers, MA, USA) were used at final concentrations of 5 μM in the cell medium.

Techniques: Activity Assay, Functional Assay, Incubation, Transfection, Binding Assay, Stable Transfection